2017-03-21 16:18:212025-05-14 02:43:03
title
ricT
yaaT
description
subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex, control of the [SW|phosphorelay], required for the achieving a sufficient level of [[protein|Spo0A]]-P for [SW|sporulation] initiation
subunit of the regulatory iron-sulfur containing [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex, required for [[protein|Rny|RNase Y]] dependent maturation of polycistronic mRNAs, control of the [SW|phosphorelay], required for the achieving a sufficient level of [[protein|Spo0A]]-P for [SW|sporulation] initiation
locus
BSU00320
BSU_00320
geneLength
825
828
function
regulation of [SW|sporulation] initiation
control of [[protein|Rny|RNase Y]] activity, see [SW|Targets of the Y complex]
names
ricT, yaaT
yaaT
ricT
outlinks
bsu
BSU00320
BSU_00320
Gene
Coordinates
41,657 -> 42,484
41,657 → 42,484
Gene
Phenotypes of a mutant
block of [SW|sporulation] at stage 0 [Pubmed|12270811]
strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]
block of [SW|sporulation] at stage 0 [Pubmed|12270811]
strongly reduced [SW|genetic competence], strongly reduced [SW|sporulation] [Pubmed|23490197]
defective in [[protein|Rny|RNase Y]]-mediated maturation of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]
The protein
[SW|Localization]
peripheral membrane, as well as to the septum, during [SW|sporulation] [Pubmed|12270811]
cytoplasm [pubmed|27501195]
cell periphery, depending on [[protein|Rny|RNase Y]] [pubmed|29794222]
Expression and Regulation
[[this]]
Biological materials
Mutant
MGNA-B899 (ricT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1898 NBRP B. subtilis, Japan]
MGNA-B899 (ricT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1898 NBRP B. subtilis, Japan]
BKE00320 (Δ[[gene|yaaT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00320 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_GACACCAATTACATTGTACA, downstream forward: _UP4_ACAGATTAACGAGGTGTGGA
BKK00320 (Δ[[gene|yaaT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACACCAATTACATTGTACA, downstream forward: _UP4_ACAGATTAACGAGGTGTGGA
References
The protein
Catalyzed reaction/ biological activity
the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex acts as specificity factor for [[protein|Rny|RNase Y]]-dependent processing of polycistronic mRNAs, see [SW|Targets of the Y complex] [pubmed|29794222]
the [[protein|YmcA]]-[[protein|YlbF]]-[[protein|YaaT]] complex stimulates [[protein|Spo0A]] phosphorylation in the [[category|phosphorelay]] [pubmed|27501195]
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
PSP1 C-terminal domain (aa 61-146) (according to UniProt)
The protein
[SW|Cofactors]
two 4Fe-4S clusters (fully co-ordinates one cluster and contributes to the co-ordination of the second (with [[protein|YmcA]] and [[protein|YlbF]])) [pubmed|31530674,28295778]
Expression and Regulation
Operons
[[this]]
Expression and Regulation
Other regulations
[[this]]
Expression and Regulation
additional information
600,000/ 11,500 molecules per cell during growth in LB/ minimal medium [pubmed|31530674]
References
Reviews
References
Original publications